VNO Atlas Figure 2

This file contains statistics and figures from the paper, “Molecular, Cellular, and Developmental Organization of the Mouse Vomeronasal organ at Single Cell Resolution” by Max Hills Jr., Limei Ma, Ai Fang, Thelma Chiremba, Seth Malloy, Allison Scott, Anoja Perera, and C. Ron Yu.

The paper can be found here:

Molecular, Cellular, and Developmental Organization of the Mouse Vomeronasal organ at Single Cell Resolution

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Novel neuronal lineage

UMAP visualization of cell-type clusters for the neuronal lineage

How many mature neurons are OSNs?

all_mature_neurons <- c("V1R_mVSN","V2R_mVSN","mOSN","sVSN")
table(neuron.integrated@active.ident)[c("mOSN")]/sum(table(neuron.integrated@active.ident)[all_mature_neurons])

      mOSN 
0.02236955 

Normalized Gap43 and Stmn2 expression in the neuronal lineage

In all lineages the cells can be distinguished to have both immature and mature populations, as determined by the expression of Gap43 and Stmn2 genes (Fig. S1).

Normalized Xist expression in the neuronal lineage, split by sex

Expression of Gnai2 and Gnao1 in the neuronal lineage

Violin plot of normalized Gnai2 expression in mature V2R neurons, split by sample

Expression of Cnga2 and Gnal in the OSN lineage

Location of mOSNs

Total number of genes, counts, and percent ribosomal gene expression detected in mature neurons, split by cell type

Location of sVSNs in a VNO slice

Heatmap of normalized expression for a select set of mutually differentially expressed genes between sVSNs and mature V1Rs, V2Rs, and OSNs

Heatmap of 503 significant (padj ≤ 0.001, fold-change ≥ 1.5) differentially expressed genes in sVSNs

[1] "sVSN Significant Upregulated Genes"
[1] 97
[1] "sVSN Significant Downregulated Genes"
[1] 406

Enriched gene ontology (GO) terms in sVSNs when compared with V1R and V2R VSNs, respectively

Box plots of normalized expression for Muc2, Obp2a, Obp2b, and Lcn3 across mature sensory neurons

Significantly regulated genes in sVSNs

The sVSNs are distinctive from the mature VSNs by a higher expression of P2ry14, Ap1s3, Cspp1, and lower expression of Omp, Tuba1a, and Ptma (Fig. 2f and S6).